Figure 2.

OTOG Expression Profile in Human Tissues

Relative OTOG mRNA levels as determined by qPCR in fetal (A) and adult (B) tissues. Because this was performed for adult and fetal tissues in two separate experiments, fetal inner ear was included in both to be able to compare the expression levels. The delta Ct method was used to calculate the expression levels. To calculate the relative expression, we set the tissue with the lowest detectable expression (Ct < 35) to 1. In the adult tissues, the expression in colon was set to 1 and in fetal tissues the expression in kidney. RNA was isolated from inner ear of an embryo at 8 weeks gestation; the other fetal RNA samples were commercially available (all adult tissues from Stratagene; fetal heart, brain, and kidney from Clontech, fetal skeletal muscle, liver, lung, spleen, thymus, colon, and stomach from Stratagene) and derived from embryos at 20–21 weeks of gestation.