Fig. 4.

Prostaglandin levels are increased by LPS. MTCC were treated with vehicle or 100 ng/mL LPS for 2, 4, or 8 h. Additionally, MTCC were pretreated with vehicle or 10 µM NS-398 for 30 min, then vehicle or 100 ng/mL LPS for 4 h. Lipid values were normalized to vehicle control for each experiment. Data was analyzed using one-way ANOVA with Newman Keuls post-hoc. * indicates a significant difference compared to vehicle control; % indicates a significant difference compared to NS-398; * indicates a significant difference compared to 2 h; # indicates a significant difference compared to 8 h. Data represents means ± SEM (normalized to vehicle controls) from at least 3 independent experiments (n=9–12, p≤0.05).