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LPS induces MAP kinase phosphory-lation and IκB-α degradation. MTCC were treated with vehicle or 100 ng/mL LPS for 0, 15, 30, 45, 60, or 120 min. (A) p38, JNK, and ERK phospho-rylation and total levels were assessed by Western blot using enhance chemiluminescense and autoradiography. (B) IκB-α and α-tubulin protein levels were evaluated by western blot. Blots are representative of at least 3 independent experiments.
