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. 2012 Nov 2;91(5):958–964. doi: 10.1016/j.ajhg.2012.10.003

Figure 3.

Figure 3

LRRC6 Localizes to the Cytoplasm and Cilia and Is Absent in DCP18

(A–F) In control cells, LRRC6 (green) localizes to the cytoplasm and within cilia, whereas it is absent from the individual’s cells. Axoneme-specific antibodies directed against α-β-tubulin were used as a control. Nuclei were stained with DAPI (blue).

Similar experiments were performed with antibodies directed against the ODA component DNAI2 (G–L) and the IDA component DNALI1 (M–R). In control cells, DNAI2 and DNALI1 localize within the cilia, whereas labeling is absent from the individual’s cells. Airway epithelial cells were examined after labeling with a mouse LRRC6 antibody (Abnova) and a secondary goat anti-mouse Alexa Fluor-488 (green) antibody (Invitrogen). For controls, we used antibodies directed against various axonemal components: α-β-tubulin (Cell Signaling Technology), DNAI2 (Abnova), and DNALI1 (Abnova) for the visualization of microtubules, ODAs, and IDAs, respectively; primary antibodies were revealed with a secondary Alexa Fluor-594 (red) or Alexa Fluor-488 (green) antibody (Invitrogen). White scale bars represent 5 μm.