Table I.

Segregation (resistance:sensitivity) of FRE-transformed T₁ populations and χ² and P values based on 3:1 segregation ratios

Line	Selective Agent	No. of Resistant Seedlings	No. of Sensitive Seedlings	χ2 (3:1)	P
FRE1-A	kana	116	33	0.647	0.42
FRE1-B	kan	105	36	0.021	0.88
FRE1-C	kan	84	25	0.248	0.62
FRE2-A	bial	87	30	0.026	0.87
FRE2-B	bial	129	44	0.017	0.90
FRE2-C	bial	90	27	0.231	0.63
FRE1+2-Ab	bial	119	46	0.729	0.39

^akan = 200 mg/L kanamycin; bial = 10 mg/L bialaphos. 

^bFRE1+2-A was derived from a homozygous T₂ of FRE1-C by transformation with FRE2.