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(A) Nuclei of control (C) and IPAH (I) PASMCs treated with MNase and profile of bulk chromatin analyzed by gel electrophoresis to allow calculation of nucleosome repeat length (NRL). S = standard. (B) Plot of nucleosome number against DNA length for bulk chromatin from control (n = 3) and IPAH (n = 3) PASMCs. Controls show a nuclear repeat length (NRL) of 171 while that of the IPAH samples show an NRL of 191. Data are mean + SEM, *P < 0.05.
