Skip to main content
. 2012 Jun 22;1(3):e000901. doi: 10.1161/JAHA.112.000901

Figure 4.

Figure 4.

The MAPK pathway is involved in Notch-mediated SMC differentiation. A, Primary human SMCs were transduced with green fluorescent protein (GFP) or Notch1ICD (N1ICD) and were treated with TSA or control vehicle dimethyl sulphoxide (DMSO) for 48 h before analysis. Phosphorylated and total forms of p38 and JNK were measured. B, GFP- or N1ICD-transduced SMCs were treated with the MEK/Erk inhibitor U0126, JNK inhibitor SP600125, or control DMSO for 48 h before analysis by immunoblot for SMC markers. Protein levels are quantified in the graph on right. C, GFP- or Notch1ICD-transduced SMCs were treated with the p38 inhibitor SB202190 or control vehicle for 48 h before immunoblot analysis. D, GFP- or N1ICD-transduced SMCs were treated with the JNK inhibitor SP600125 (SP), the p38 inhibitor SB202190 (SB), or control vehicle DMSO (con) for 48 h, and total RNA was collected for expression analysis by quantitative RT-PCR for SM22α and CNN1. Data are presented as fold change compared to SMCs with control treatment. Data are presented as mean±SEM and were statistically analyzed by ANOVA / Tukey test. Asterisks indicate P<0.05; both JNK and p38 inhibitors significantly suppressed N1ICD induction of these genes.