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Assessment of apoptosis in p4 and p21 ECs. Mouse aortic ECs were serially passaged from p4 to p21, and apoptosis was assessed by Hoechst nuclear staining (A), Bax/Bcl-2 ratio (B), and caspase-3 activity (C). Inset: representative blot. Data are expressed as mean±SEM; *P<0.05 vs p4 cells, n=5 to 6.
