Figure 11.

24(S),25-EC, T0901317, and OSCi increase SREBP-1c mRNA in human SMCs, but only T0901317 increases FA and triglyceride synthesis. A and B, After 24-h preincubation in M199 media containing 0.4% FBS, SMCs were incubated (24 h) in M199 0.4% FBS media with or without vehicle, 24(S),25-EC, T0901317, or OSCi. Values for mRNA of SREBP-1c (n=4) (A) and FASN (n=3) (B), normalized to GAPDH, are mean±SEM relative to control. *P<0.001 vs control, as analyzed by the Mann-Whitney U nonparametric test. C, SMCs were treated as in A and B. Representative immunoblots are of precursor (p) and nuclear (n) SREBP-1 from SMC postnuclear and nuclear fractions, respectively (n=3). The nSREPB-1/Lamin A/C blots in C (right) were derived from gels run for a longer time (≈180 min) compared to the other nSREPB-1 / Lamin A/C blots (≈120 min) (left and center). D, [¹⁴C]acetate incorporation into FA (5 h) in SMCs preincubated as in A and B. Values were normalized to control for 4 separate experiments (n=4) and are mean±SEM. *P<0.008 vs control as analyzed by the Mann-Whitney U nonparametric test. E, [¹⁴C]oleate incorporation into triglyceride (5 h) in SMCs incubated as in D. Values are normalized to control and are mean±SEM for 5 separate experiments (n=5). *P<0.05 vs control, as analyzed by Student t test.