Figure 4.

Inhibition of iNOS with L‐NAME affected LPS‐induced production of only IL6 but not of any other proinflammatory mediators in macrophages. qRT‐PCR analysis of LPS‐induced (0.1 μg/mL, 22 h) mRNA expression of proinflammatory mediators in the absence or presence of the NOS inhibitor L‐NAME (5 mmol/L) in bone marrow–derived macrophages from wild‐type mice. Nontreated cells were used as control (C). Experiments were performed on day 7 after differentiation ex vivo. Cells were serum‐starved for 4 h before addition of L‐NAME (pretreatment for 2 h), followed by addition of LPS. Data shown are mean±SEM from 10 individual animals. *P<0.05, **P<0.01, and ***P<0.001 vs control or between indicated groups.