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. 2012 Aug 24;1(4):e000992. doi: 10.1161/JAHA.112.000992

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© 2012 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley-Blackwell.

This is an Open Access article under the terms of the Creative Commons Attribution Noncommercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Figure 10. — Targeted disruption of Arg‐II reduced atherosclerosis in ApoE^−/− mice. Mice were fed either HF (A and B) or HC (C, D, and E) diet for 10 weeks. A, Representative images showing Oil Red O staining of plaques in aortic roots of ApoE^−/−Arg‐II^+/+ and ApoE^−/−Arg‐II^−/− mice. Quantifications of the lesions are presented in the graph below the stains. Data shown are medians with 25th and 75th percentiles from 8 animals of each group. At least 7 equally spaced cryosections of aortic roots per mouse were evaluated. B, Representative confocal microscopic images showing macrophage accumulation in the lesions stained with antibodies against F4/80 (red), or CD11c (green) and MMP14 (red). All sections were counterstained with DAPI (blue). The merged images are shown. Scale bars=10 μm. Quantifications of the positive stained cells are presented in the corresponding graphs on the right. Data shown are medians with 25th and 75th percentiles from 8 animals of each group. C, Representative images of Oil Red O staining of lesions in thoracic‐abdominal aortas. Quantifications of the lesions are shown in the graph below the stain (n=18 of each group). D, Hematoxylin‐eosin staining of aortic arches of the 2 mouse groups. Quantifications of necrotic core (indicated by #) are presented as percentage of the cell‐free area to the total lesion area and are shown in the graph below the stain (n=10). At least 4 equally spaced sections per mouse were evaluated. E, qRT‐PCR analysis of F4/80, MMP14, TNFα, and IL6 in the plaques isolated from aortic arches. Data shown are mean±SEM from 10 animals of each group. F, In vivo adoptive transfer. Labeled monocytes from donor ApoE^−/−Arg‐II^+/+ (sk: groups 1 and 2) and ApoE^−/−Arg‐II^−/− (dk: groups 3 and 4) mice were injected into recipient ApoE^−/−Arg‐II^+/+ (groups 1 and 3: sk→sk and dk→sk, respectively) and ApoE^−/−Arg‐II^−/− (groups 2 and 4: sk→dk and dk→dk, respectively) mice. Data are presented as percentage change to the sk→sk control group. The number of fluorescent macrophages counted in atherosclerotic lesions of the control group (sk→sk) is 61.29±11.46 (n=7 for groups 1 and 3; n=6 for groups 2 and 4). *P<0.05; ***P<0.001 between the indicated groups.