Figure 1.
In vitro efficacy of Ad-siRNA-Gαs gene transfer. Gαs protein expression was analyzed using Western blot in HL-1 mouse atrial cardiac myocytes. (A) Gαs protein levels evaluated in untreated control cells and following application of Ad-GFP (GFP) or Ad-siRNA-Gαs (siRNA). (B) Quantification of optical density normalized to GAPDH protein. Gαs expression was suppressed by 51% in HL-1 cells infected with Ad-siRNA-Gαs (n=3) compared with controls (n=3), whereas Ad-GFP did not significantly alter Gαs protein levels (n=3). Data are provided as mean±SEM; ***P<0.001 versus control HL-1 cells. GAPDH indicates glyceraldehyde-3-phosphate-dehydrogenase; GFP, green fluorescent protein.