Figure 1.

Role of ADP in megakaryocyte differentiation and platelet release. (A) ADP and ATP were constitutively released into the conditioned medium during megakaryocyte differentiation in culture (means±SD, n=5 separate experiments. (B) On day 13 of maturation, cord blood derived-megakaryocytes were seeded for 16 h in the presence or absence of the ADP scavengers apyrase (1 U/mL) or CP/CPK (5 mM/40 U/mL), and proplatelet formation was quantified (mean±SD, n=5 separate experiments. (C) Megakaryocytes on day 13 of culture were incubated for 16 h with the P2Y₁ inhibitor MRS 2179 (10 μM), the P2Y₁₂ inhibitors cangrelor (10 μM) and 2-MeSAMP (10 μM), or the active metabolites of clopidogrel (clopidogrel-AM, 10 mM) or prasugrel (prasugrel-AM, 10 μM) and proplatelet formation was quantified (mean±SD, n=5 separate experiments). *P<0.05 compared to untreated control (CTRL), MRS 2179, clopidogrel-AM and prasugrel-AM treated samples (ANOVA and Bonferroni's t-test as a post-hoc test).