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Akt and ERK1/2 phosphorylation by proplatelet-forming megakaryocytes in culture is inhibited by MRS 2211. Western blot analysis of (A) Akt and (B) ERK1/2 phosphorylation in human megakaryocytes on day 13 of culture in the presence (+) or absence (−) of the specific P2Y13 inhibitor MRS 2211 (10 μM). Samples were also probed with anti-Akt, anti-ERK1/2, anti-CD61 and anti–β-actin antibodies (representative of 3 experiments).
