Figure 1.

Sensitivity of FLT3-ITD mutants to the AC220 inhibitor. Ba/F3 cells expressing FLT3-ITD (W51 mutation as described by Kelly et al.¹¹), FLT3-D835Y and different FLT3-ITD mutants were treated with increasing concentrations of AC220 and their proliferation was measured over a period of 24 h (A). (B) The same cells were also harvested for Western blot analysis after treatment for 90 min with different concentrations of AC220. The phosphorylation of the different FLT3 proteins was determined using a phospho-FLT3 antibody (Tyr591/3466, Cell Signaling Technology).