Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Magn Reson. 2012 Apr 26;220:79–84. doi: 10.1016/j.jmr.2012.04.006

Figure 1.

Figure 1

(A) Pulse sequence used for optimizing SIM-CP (simultaneous cross polarization) parameters (B) 2D DUMAS-NCA-CAN pulse sequence using a bidirectional specific-CP transfer preceded by 13C and 15N parallel evolutions. 13C and 15N are detected in separate experiments. The φ2, φ3 phase cycle allows the magnetization from 15N to 13Cα and vice versa. (C) DUMAS pulse scheme for simultaneous acquisition of NCACX and CANCO 3D experiments. The evolution of t1′ and t1″ are incremented in parallel, similarly t2′ is incremented in parallel with t2″. The dwell time of t1′ and t2″ are twice to t1″ and t2′. The phase of φ* is shifted between 0° and 90° for states mode acquisition of the indirect dimensions. (D) DUMAS scheme for simultaneous acquisition of NCOCX and CON(CA)CX 3D experiments. φ1 = (y, −y)4; φ2 = (y, y, y, y, −y, −y, −y, −y); φ3=(x, x, −x, −x)2; φrec=(x, −x, −x, x, −x, x, x, −x).