In vitro effects of VIP treatment on ECM protein levels from B6- and BALB/c-derived fibroblasts. Fibroblasts were stimulated with LPS (25 μg/mL) + VIP treatment (10−9 M) for 18 hours. Protein levels of ITG-α1 (A), CD44 (B), LAMA-1 (C), ECM1 (D), VCAN (E), and SPP1 (F) were determined by ELISA.
* ITG-α1: P < 0.001 (all groups); CD44: P = 0.002, = 0.001, = 0.04, = 0.03; LAMA-1: P < 0.001 (all groups); ECM1: P = 0.041, = 0.028; VCAN: P = 0.016, = 0.037; SPP1: P < 0.001 (all groups).