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TGF-β2 induces FST protein expression in primary cultured primary TM cells assessed by immunostaining. DAPI staining of nuclei (A, D). FST staining in human TM cells treated without (B) or with (E) TGF-β2 (5 ng/mL) for 48 hours. Merge of FST staining and DAPI in control (C) and TGF-β2–treated cells. (F) FST staining was increased in the perinuclear region (arrowheads) in the TM cells. Scale bar = 100 μm.
