Figure 4.
RNA gel-blot hybridization analysis for various members of the tydc gene family in mature opium poppy organs. Fifteen micrograms of total RNA was fractionated on 1.0% formaldehyde agarose gels, transferred to nylon membranes, and hybridized at high stringency with 32P-labeled full-length probes for tydc1 and tydc2 or gene-specific probes for tydc1/8, tydc2/7, tydc3, tydc4, tydc6, and tydc9. To ensure equal loading, gels were stained with ethidium bromide before blotting.
