Table II.

Oligonucleotide primers used to amplify and subclone 5′ flanking regions from tydc3, tydc6, tydc7, tydc8, and tydc9

Name	Sequencea	Product Sizeb
		kb
TYDC3-3c	GGG GGG GGA TCC GAA GAA GAA AGA GAG GTG GT	3.5
TYDC3-4	GGG GGG AAG CTT CTG TGT GCC AAC CCG CGA TA
TYDC6-3	GGG GGG GGA TCC TTG CTG ATT AGT GAG GGA GA	3.0
TYDC6-4	GGG GGG AAG CTT ATA GAA GTT GTT GGG AGA TA
TYDC7-3	GGG GGG CTC GAG CAG GTG AAA GAA GGT TAT TG	1.2
TYDC7-4	GGG GGG AAG CTT TTA TCC ACA CCC AAC TCA TC
TYDC8-3	GGG GGG GGA TCC CGT TAC TAT CAG TTT TGA TG	1.2
TYDC8-4	T3 primerd
TYDC9-3	GGG GGG GGA TCC TGT TAC TGG TTT TGC TAA TG	2.1
TYDC9-4	GGG GGG GTC GAC CAA ATG AGG ACC CAA ATC TG

^aUnderlined sequences represent restriction endonuclease sites introduced into each primer to allow efficient cloning of the PCR product into the appropriate vector. 

^bThe product size is that resulting from PCR using the matching set of sense and antisense primers with the corresponding template. 

^cDesignation of the oligonucleotide as “-3” indicates the sense primer, whereas “-4” indicates the antisense primer. 

^dThe antisense primer used to amplify the 5′ flanking region of tydc8, subcloned into the XhoI site, was the standard pBluescript T3 primer. The HindIII site from the pBluescript polylinker was used for ligation of the PCR product.