Figure 3.

Multipotent differentiation of GFP-ADSCs. (A) Alizarin Red detected calcium mineralization in osteogenic cultures of GFP-ADSCs for 3 weeks in vitro. (B) Oil Red O staining detected red-colored oil granules in adipogenic cultures of GFP-ADSCs (magnified view of the square area in [B]). (C and D) For chondrogenic differentiation, histological and hematoxylin and eosin staining results showed that cartilage lacunae were formed and expressed chondrocyte gene markers, collagen II (C and D).

Notes: Scale bar A measures 25 μm, B, C, and D measure 100 μm.

Abbreviation: GFP-ADSCs, green fluorescent protein adipose-derived stem cells; HE, hematoxylin and eosin.