Figure 4.
RHAMM expression before and after transplantation of human cord bloodderived CD34+ cells in NOD/SCID mice. Eight adult NOD/SCID mice were intravenously injected with 2.5×105 cord blood-derived CD34+ cells. (A) Plots are gated on the viable cells, and show the sorting strategy. Dot plots illustrate a representative mouse 2 weeks after transplantation of hCD34+ cord blood-derived cells. The percentage of hCD45+ mCD45- hCD34+ cells within the viable bone marrow cells is 5.79%. (B) Box plot showing the results of RT-qPCR analysis for RHAMM mRNA in freshly isolated CD34+ cells (pre-injection= pre) and in CD34+ cells isolated from the bone marrow 2 weeks after intravenous injection (post). Results were normalized to the geometric mean of the expression of GAPDH, R18S and YWHAZ, and are shown relative to the RHAMM transcript level in K562 cells. There is a significant difference in RHAMM expression level between the ‘pre’ and ‘post’ samples (P=0.012 calculated by Wilcoxon’s test), with firm up-regulation of RHAMM mRNA 2 weeks after transplantation.