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. 1998 Sep;118(1):83–90. doi: 10.1104/pp.118.1.83

Figure 2.

Figure 2

A, Cross-section of developing muskmelon seeds at 25 DAA. The seeds were decoated carefully, sectioned, and stained with aniline blue and Sudan III and IV. Only Sudan staining (arrow) was detected on the endosperm envelope. B, Freeze-fractured cross-section of endosperm envelope 25 DAA viewed by SEM. Only the plate-like or waxy appearance was evident; the globular layer present in mature envelopes was absent. C, Cross-section of developing muskmelon seeds at 35 DAA. Decoated seeds were hand sectioned and stained with aniline blue. Aniline-blue-staining vesicles were observed in the endosperm envelope. These vesicles were not present in other developmental stages. D, Cross-section of mature muskmelon seeds (55 DAA). Decoated seeds were hand sectioned and stained with aniline blue and Sudan III and IV. The orange Sudan-staining lipid layer (arrow) was observed on top of the thick, aniline-blue-staining layer. The bar in each panel represents 5 μm.