Fig. 3.

Characteristics of AR and ERα binding sites in ZR-75–1 cells defined by ChIP-seq. ChIP validation of ERα (panel A) and AR-binding sites (panel B) in independent sample sets at different peak stringency thresholds. Data were calculated as percentage input and presented as fold enrichment with E₂ or DHT over vehicle. The x-axis demarcates ChIP site number, and annotations above the bars represent the gene nearest to each site. Color coding of gene names between panels A and B indicates genomic regions common to both AR and ERα binding, whereas those in black were unique to each set. C, Conservation plot of ZR-75–1 AR and ERα cistromes in vertebrate species. Average PhastCons scores are based on alignment of binding sites with homologous gemonic regions from chimpanzee, mouse, rat, dog, chicken, fungi, and zebrafish. The center of each binding site was set as zero. D, Genomic location of AR- and ERα-binding sites (numbers are percent of sites). E, Distribution of AR- and ERα-binding sites within 100 kb upstream or downstream of annotated transcription start sites. THOC5, THO complex 5; SND1, staphylococcal nuclease and tudor domain containing 1; EEPD1, endonuclease/exonuclease/phosphatase family domain containing 1; COL28A1, collagen, type XXVIII, alpha 1; HAPLN2, hyaluronan and proteoglycan link protein 2; GTF3, general transcription factor 3.