Table 2.
3D-microCT analysis of femur distal metaphysis trabecular structure and midshaft cortical envelope
| WT | Dmp1ko | Dmp1ko/Dmp1Tg(full length) | Dmp1ko/Dmp1Tg(57 kDa) | |
|---|---|---|---|---|
| Trabecular bone parameters | ||||
| Tb.BMD (mg HA/cm3) | 149.9 ± 11.3 | 2.3 ± 0.8a | 29.8 ± 3.1a,b | 68.4 ± 13.8a,b |
| BV/TV (%) | 21.2 ± 1.2 | 0.7 ± 0.2a | 7.1 ± 3.1a,b | 11.5 ± 2.1a,b |
| Tb.N. (−1) | 5.2 ± 0.2 | 1.8 ± 0.1a | 2.7 ± 0.4a,b | 4.0 ± 0.2a,b |
| Tb.Th (μm) | 56.9 ± 3.1 | 38.1 ± 1.0a | 45.6 ± 4.7b | 49.3 ± 4.9b |
| Tb.Sp (μm) | 188.6 ± 8.4 | 557.6 ± 27.7a | 280.6 ± 51.3a,b | 239.1 ± 17.7a,b |
| Conn.Dens. | 231.8 ± 17.8 | 1.7 ± 1.0a | 74.0 ± 28.5a,b | 116.7 ± 15.3a,b |
| SMI | 1.7 ± 0.1 | 3.5 ± 0.1a | 2.4 ± 0.1a,b | 2.4 ± 0.2a,b |
| DA | 1.89 ± 0.05 | 1.81 ± 0.06 | 1.82 ± 0.07 | 1.85 ± 0.03 |
| Cortical bone parameters | ||||
| Ct.BMD (mg HA/cm3) | 862.0 ± 8.3 | 549.2 ± 16.6a | 809.3 ± 32.5a,b | 797.8 ± 15.5a,b |
| Ct.Th. (μm) | 146.2 ± 7.3 | 50.1 ± 2.3a | 133.9 ± 3.4b | 118.7 ± 4.5a,b |
| CSA (mm2) | 2.31 ± 0.09 | 2.47 ± 0.09 | 2.48 ± 0.01 | 3.03 ± 0.12a,b |
| Ct.Ar. (mm2) | 0.60 ± 0.03 | 0.18 ± 0.02a | 0.59 ± 0.01b | 0.55 ± 0.03b |
| Ma.Ar. (mm2) | 1.73 ± 0.06 | 2.40 ± 0.14a | 1.89 ± 0.02 | 2.33 ± 0.14a |
Values are expressed as mean ± se from five mice per group at 5 wk of age. Comparisons were performed using one-way ANOVA and post hoc Fisher test. Tb.BMD, Trabecular bone mineral density; Conn.Dens, connectivity density; DA, degree of anisotropy; Ct.BMD, cortical bone mineral density; Ct.Th, cortical thickness; CSA, cross sectional area; Ct.Ar, cortical area; Ma.Ar, marrow area.
a
P < 0.05 vs. WT.
b
P < 0.05 vs. Dmp1ko.