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. 2012 Nov 2;7(11):e47545. doi: 10.1371/journal.pone.0047545

Figure 1. Induction of Reg IV expression by CDX2.

Figure 1

A: Western blot analysis of CDX2, Reg IV, and β-actin and qRT-PCR analysis of REG4 in 9 GC cell lines. B: Western blot analysis of CDX2, Reg IV, and β-actin and qRT-PCR analysis of REG4 in HT-29/PGS-CDX2, HT-29/PGS-neo, SW480/PGS-CDX2, and SW480/PGS-neo. C: Western blot analysis of Reg IV and β-actin and qRT-PCR analysis of REG4 in HT-29/CDX2-ER. Time course of REG4 gene induction in response to activation of a CDX2-ER fusion protein by 4-OHT was analyzed. D: Western blot analysis of CDX2, Reg IV, and β-actin and qRT-PCR analysis of REG4 in HSC-39 cells transfected with CDX2 siRNA (siRNA1 and siRNA2) and the negative control siRNA. The units of REG4 mRNA expression level are arbitrary. P values were calculated using Student’s t-test. * N.S. = not significant.