FIG 7.

Guanidine HCl prevents cleavage and relocalization of AUF1 during poliovirus infection. (A) HeLa cells were mock treated (lanes 1 to 6) or treated with 2 mM guanidine HCl (GuaHCl; lanes 7 to 12) during mock infection (lanes 1 and 7) or poliovirus infection (lanes 2 to 6 and lanes 8 to 12). Lysates were collected at indicated hours postinfection (hpi) and subjected to SDS-PAGE and Western blot analysis using anti-AUF1 and antinucleolin (as a loading control) antibodies to visualize proteins. Electrophoretic mobilities of nucleolin and AUF1 are indicated to the right by an arrow and a bracket, respectively. (B) HeLa cells seeded on coverslips were mock infected or infected with poliovirus and treated with 2 mM guanidine HCl. Cells were fixed at either 0 or 5 hpi, immunostained with anti-AUF1 antibody (in red) and anti-3A antibody (in green), and stained with DAPI (blue) to visualize the nucleus. Proteins were visualized using a Zeiss Axiovert 200 M inverted microscope.