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PKCϵ activity is rapidly stimulated by NTHi.A) Bovine bronchial epithelial cells (BBECs) were exposed to 0, 10, 25, 50, and 100% NTHi for 1–120 minutes. The 50% and 100% NTHi rapidly stimulates PKCϵ within 1 minute. PKCϵ activity returns to baseline after 2 hours (p < 0.05 Media vs. 50% from 1–60 min. p < 0.01 Media vs. 100% from 1–60 min) (n = 6). B) PKCϵ activity remains auto-downregulated at 24 hours (*p < 0.001 vs. Media) (n = 6).
