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NTHi supernatant decreases the total number of motile points after 24–48 hours of exposure. BBECs were exposed to 50% NTHi supernatant and 50% NTHi growth media alone (Brain-heart infusion broth) for 1–48 hours. The total number of motile points was measured. At 24 and 48 hrs, a significant decrease in motile cilia was detected (*p < 0.01) in the ciliated cells exposed to NTHi supernatents (n = 8).
