Table 2. Genes involved in eDNA release identified by the transposon library mutagenesis.

Number of clones	Gene	Insertion	Function
12	oppA	CGCTTGACC	Oligopeptide permease, involved in quorum sensing, sporulation and competence
1	oppF	GCTCACATA	ATP binding protein of the Opp transporter (quorum sensing, sporulation, competence)
1	Deletion comX-comP	From AAAGCTTAT (comX) to TGCTGAGCA (comP)	ComX, pheromone involved in competence. ComP, histidine kinase sensor (two component system), phosphorilation of ComA, competence
3	comP	AACTTTGAC GGTGTAACT GGTCAAGCA	ComP, histidine kinase sensor
1	degU	GTTGTGGTC	Competence regulator (two component system). Essential for expression of ComK (regulator required for the transcription of genes involved in DNA uptake and integration. Also involved in motility
2	mfd	GACCCTTAC TGCAGGCTA	DNA repair (displaces RNA polymerase stalled at a lesion). DNA homologous recombination
1	topA	TGTGAAGTG	DNA topoisomerase I
2	yqhG	GGCCCCGCG	unknown