Figure 8.

Effect of SIRT2 depletion on the reversion of RAF-induced senescence after cyclin-dependent kinase inhibitor (CKI) knockdown. WI-38hTERT/GFP-RAF-ER cells were transfected (24 hours) and induced into senescence by treatment with 20 nM 4-HT for 3 days. Senescent cells were then transfected with the indicated siRNAs and cells were incubated for a further 3 days before testing their capacity to incorporate 5-bromo-2'-deoxyuridine (BrdU) during an additional 24-hour incubation. Error bars show SD of two biological replicates.