TABLE 2.
FISH detection of L. reuteri in gastric and intestinal biopsy specimensa
| Subject group | Biopsy specimen |
L. reuteri detected by:
|
|||||
|---|---|---|---|---|---|---|---|
| FISH
|
Biopsy plating
|
Fecal plating
|
|||||
| Day 0 | Day 28 | Day 0 | Day 28 | Day 0 | Day 28 | ||
| Gastroscopy | Corpus | 1 of 10 | 7 of 10 | 2 of 10 | 1 of 10 | 0 of 10 | 10 of 10 |
| Antrum | 1 of 10 | 8 of 10 | 3 of 10 | 1 of 10 | |||
| Duodenum | 3 of 10 | 10 of 10 | 3 of 10 | 0 of 10 | |||
| Ileoscopy | Ileum | 3 of 6 | 6 of 6 | 4 of 9 | 1 of 9 | 2 of 9 | 6 of 9 |
a
Biopsy and fecal or ileostomy output samples were taken from subjects at baseline (day 0) and after the daily administration of L. reuteri ATCC 55730 at 4 × 108 CFU/day for 28 days (day 28). L. reuteri was detected in biopsy specimens by either FISH or plating using classical methods. Results are expressed as the number of subjects in which L. reuteri was detected out of the total number of subjects analyzed. Examples of FISH detection are shown in Fig 1.