Table 2.

Primers used for PCR

Gene	Sequence of primers		Size of PCR(bp)	Denaturation temperature (°C)	Cycles
ASK1	sense	5’-TTCACACAAAACGGATGTAACATT-3’	198	56	30
	antisense	5’-CCTAAACAGTTATGGTCACATTTTGG-3’
Claudin-6	sense	5’-TTCATCGGCAACAGCATCGT-3’	345	58	35
	antisense	5’-GGTTATAGAAGTCCCGGATGA-3’
GAPDH	sense	5’-TGTTGCCATCAATGACCCCTT-3’	202	56	25
	antisense	5’-CTCCACGACGTACTCAGCG-3’