Table 1.
Numbers of essential genes under laboratory conditions in relevant E. coli, S. Typhimurium and S. Typhi isolates
Species/serovar | Strain | Essential genes/non- essential | Method (type of mutagenesis, medium) | Reference |
---|---|---|---|---|
E. coli |
K-12 MG1655 |
302/4477 |
Published literature and MD (medium-scale) and LD (large-scale) deletion mutants (targeted mutagenesis, antibiotic medium 3) |
Profiling of E. coli chromosome (PEC) database (
http://shigen.lab.nig.ac.jp/ECOli/pec/)
[9,10] |
E. coli |
K-12 BW25113 |
303/3985 |
Single-gene deletion mutants (targeted mutagenesis, LB) |
Keio collection
[7] |
E. coli |
K-12 BW25113 |
299/3864 |
Single-gene deletion mutants (targeted mutagenesis, LB) |
Update on the Keio collection
[8] |
E. coli |
K-12 W3110 |
299/4109 |
Published literature |
PEC database (
http://shigen.lab.nig.ac.jp/ECOli/pec/)
[9,10] |
S. Typhimurium |
ATCC 14028 |
NA/1,023 |
Single-gene deletion mutants (targeted mutagenesis, LB) |
[4] |
S. Typhimurium |
ATCC 14028 |
257/NA |
Insertion-duplication mutagenesis (IDM) sequencing (random mutagenesis, LB) |
[5] |
S. Typhimurium |
LT2 |
144 (LB and/or M9/glc)/NA |
Metabolic reconstruction (in silico approach, M9/glc and LB) |
[6] |
S. Typhi | Ty2 (STY2) | 356/4162 | Random transposon mutagenesis and two types of growtha | [3] |
a Plating on an “aro mix” agar containing L-phe, L-trp, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid (condition 1), six passages of growth in Luria broth (condition 2).