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. 2012 May 30;13:212. doi: 10.1186/1471-2164-13-212

Table 1.

Numbers of essential genes under laboratory conditions in relevant E. coli, S. Typhimurium and S. Typhi isolates

Species/serovar Strain Essential genes/non- essential Method (type of mutagenesis, medium) Reference
E. coli
K-12 MG1655
302/4477
Published literature and MD (medium-scale) and LD (large-scale) deletion mutants (targeted mutagenesis, antibiotic medium 3)
Profiling of E. coli chromosome (PEC) database ( http://shigen.lab.nig.ac.jp/ECOli/pec/) [9,10]
E. coli
K-12 BW25113
303/3985
Single-gene deletion mutants (targeted mutagenesis, LB)
Keio collection [7]
E. coli
K-12 BW25113
299/3864
Single-gene deletion mutants (targeted mutagenesis, LB)
Update on the Keio collection [8]
E. coli
K-12 W3110
299/4109
Published literature
PEC database ( http://shigen.lab.nig.ac.jp/ECOli/pec/) [9,10]
S. Typhimurium
ATCC 14028
NA/1,023
Single-gene deletion mutants (targeted mutagenesis, LB)
[4]
S. Typhimurium
ATCC 14028
257/NA
Insertion-duplication mutagenesis (IDM) sequencing (random mutagenesis, LB)
[5]
S. Typhimurium
LT2
144 (LB and/or M9/glc)/NA
Metabolic reconstruction (in silico approach, M9/glc and LB)
[6]
S. Typhi Ty2 (STY2) 356/4162 Random transposon mutagenesis and two types of growtha [3]

a Plating on an “aro mix” agar containing L-phe, L-trp, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid (condition 1), six passages of growth in Luria broth (condition 2).

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