Skip to main content
. 2012 May 30;13:212. doi: 10.1186/1471-2164-13-212

Table 2.

Essential genes in E. coli that are not as strongly selected in Typhimurium or Typhi*

S. Typhimurium 14028 gene symbol Gene name Gene description Best hit in S. Typhimurium LT2 Best hit in S. Typhi Ty2a Best hit in ECOa STM1 transposons STM1 reads STY1 transposons STY1 reads STY2 transposonsb STY2 readsb STY2 transposonsc STY2 readsc
 
 
 
 
 
 
Percentile rank
STM14_0106
folA
Dihydrofolate reductase
STM0087
t0090
b0048
4
3
8
8
19
19
46
60
STM14_0217
folK
2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase
STM0183
t0191
b0142
12
22
18
16
25
56
16
21
STM14_2754
yejM
Putative hydrolase
STM2228
t0626
b2188
14
16
30
25
13
19
17
20
STM14_4041d
degS
Serine endoprotease
STM3349
t3265
b3235
50
34
14
14
6
7
4
4
STM14_4193
trpS
Tryptophanyl-tRNA synthetase
STM3481
t4024
b3384
18
16
14
14
78
64
12
12
STM14_4208
yrfF
Intracellular growth attenuator protein
STM3495
t4011
b3398
1
4
19
19
70
42
12
27
STM14_4460
gpsA
NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
STM3700
t3819
b3608
10
10
14
17
45
46
8
11
STM14_4921
ftsN
E cell division protein
STM4093
t3525
b3933
42
38
14
15
20
27
15
15
STM14_4971.J murB UDP-N- acetylenolpyruvoylglucosamine reductase STM4136 t3489 b3972 42 48 26 22 8 24 13 21

* Details for all genes can be found in Additional file 5: Table S2. Genes were ranked by the number of transposon insertions and number of independent reads per gene. Ranks above the 15th percentile are shown in bold.

a Best hits are listed only if gene is syntenic with S. Typhimurium strain 14028 and has at least 95% sequence identity.

b Growth on L-agar with “aro mix” (L-phe, L-trp, p-aminobenzoic acid and 2,3-dihydroxybenzoic acid) [3].

c Growth after six passages in LB broth [3].

d Considered essential in STY2 [3].

HHS Vulnerability Disclosure