Figure 1.

Kinetics of TNP-specific Ig titres following primary and boost immunisations with TNP-conjugated antigens. Five calves were subcutaneously immunised with 3 mg TNP-CGG or TNP-AECM-FICOLL in QuilA then boosted 29 days later with the same immunising antigens. The dotted vertical line indicates when the booster TNP-CGG/TNP-AECM-FICOLL immunisations were given (day 29 pi). Blood samples were taken at various time-points following these injections and TNP-specific IgG (diamond symbols) and IgM (square symbols) titres were determined by ELISA in calves from the T-D group (open symbols) or T-I group (filled symbols). Results are expressed as the mean of determinations from individual calves in each group ± SEM, n = 5.