Figure 2.

TNP-specific plasma and memory B cell detection post-boost immunisation with TNP-CGG (T-D group). Five calves were subcutaneously immunised with 3 mg TNP-CGG in QuilA then boosted 29 days later with the same immunising antigen. The dotted vertical line indicates when the booster TNP-CGG immunisation was given (day 29 pi). Blood samples were taken at various time-points following the immunisations. TNP-specific IgG, plasma cell (filled circles, left-axis) and memory B cell (open circles, right-axis) numbers were determined by B cell ELISPOT. Results were expressed as ASC per 10⁶ PBMC for plasma cells and induced ASC per 10⁶ cultured PBMC for memory B cells (mean of determinations from individual calves in each group, n = 5).