Figure 7 .

Localization of endocytic vesicles by FM4-64 staining in tobacco zygotes and proembryos. (A₁-H₁) The fluorescence images of living zygotes and proembryos stained by FM4-64. In a zygote, the fluorescence of FM4-64 evenly distributes in the whole cytoplasm (A₁). During cell plate formation, endocytic vesicles mainly localize in the new cell plate edges (B₁-D₁). After treating with 50 μM β-GlcY, the fluorescence of FM4-64 evenly distributes in cytoplasm of in vitro cultured proembryos (E₁ and F₁). In proembryos cultured without β-GlcY, the fluorescence images are similar to that of in vivo proembryos (G₁). (A₂-H₂) Bright-field images of zygotes and proembryos corresponding to A₁-G₁. (A₃-H₃) Fluorescence images of DAPI-stained nuclei corresponding to bright-field images. Bars = 10 μm.