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. 2012 Jun 20;12:111. doi: 10.1186/1471-2180-12-111

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Copyright ©2012 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Detection of the relative transcript levels of fimA , fimZ , fimY , and fimW genes using quantitative RT-PCR. The mRNA transcript levels of the major fimbrial subunit gene fimA (panel A), fimZ (panel B), fimY (panel C), and fimW (panel D) in the parental LB5010, Δstm0551, Δstm0551 (pSTM0551), and Δstm0551 (pACYC184) strains were detected by quantitative RT-PCR. The mRNA transcript levels were obtained by delta-delta Ct (ΔΔCt) method, and the expression levels (2^-ΔΔCt) of the parental LB5010 strain cultured on LB agar were set to 1 fold for each gene tested. The asterisk indicated that the differences in transcript levels were statistically significantly (p<0.05).