Table 1.

Bacterial strains and plasmids used in this study

Name	Description a	Reference or source
Salmonella enterica serotype Typhimurium
LB5010	Wild type S. enterica serotype Typhimurium LT2 strain, fimbriate with the complete fim gene cluster	[21]
Δstm0551	stm0551 deletion mutant; Kanr	Present study
Escherichia coli strain
One Shot® TOP10 chemically competent E. coli	F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG	Invitrogen
BL21Star™ (DE3) One Shot® chemically competent E. coli	F-ompT hsdSB(rB- mB-) gal dcm (DE3)	Invitrogen
Plasmids
pSTM0551	A 0.5-kb DNA fragment possessing the stm0551 coding sequence cloned into the pACYC184 vector; Cmr	Present study
pSTM0551E49A	A 0.5-kb DNA fragment possessing the stm0551 coding sequence with glutamic acid (E) at position 49 replaced with an alanine (A) cloned into the pACYC184 vector; Cmr	Present study
pACYC184	Tetr, Cmr, cloning vector; w/p15A ori	ATCC, Manassas, VA
pET101/D-TOPO	Ampr, 6xHis tag expression vector	Invitrogen
pKD46	Ampr, express λ Red recombinase system, temp- sensitive replicon	[22]
pKD13	Plasmid carrying Kanr cassette	[22]

^aAmp^r ampicillin resistant; Cm^r chloramphenicol resistant; Kan^r kanamycin resistant; Tet^r tetracycline resistant