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. 2012 Oct 24;6:20. doi: 10.1186/1754-1611-6-20

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Copyright ©2012 Mukherjee et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SDS PAGE analysis for wild type and mutant FbFPs. Lane 1: F37W, Lane 2: F37E, Lane 3: F37Y, Lane 4: F37T, Lane 5: F37S, Lane 6: Wild type FbFP. Selected mutants (F37W, F37E, F37Y, F37S, and F37T) of the FbFP F37X saturation mutagenesis library were purified and run on a 10% denaturing polyacrylamide gel (160 V, 45 minutes). Fluorescence enhanced mutants FbFP F37S and F37T comigrate with the wild type band (WT) at an approximate molecular mass of 17 kDa. Additional mutants correspond to neutral (F37E, lane 2) or deleterious mutants (F37W, lane 1 and F37Y, lane 3).