Table 2.
Classification of mutants
| Mutation | Beneficial | Deleterious | Neutral |
|---|---|---|---|
| F37X |
2 |
71 |
103 |
| D52X |
0 |
42 |
138 |
| A53X |
0 |
60 |
57 |
| R54X |
0 |
28 |
152 |
| Q57X |
0 |
30 |
150 |
| R70X |
0 |
18 |
120 |
| N85X |
0 |
141 |
20 |
| W94X |
0 |
15 |
58 |
| Y112X | 0 | 31 | 149 |
Mutations were introduced in the wild type FbFP by site saturation mutagenesis and mutants were classified as beneficial, neutral, or deleterious based on their fluorescent properties. Specifically, beneficial mutants exhibit at least a twofold enhancement in brightness of fluorescence emission (or a 10 nm or greater spectral shift), deleterious mutants have substantially diminished (greater than twofold) fluorescence emission compared to the wild type FbFP, and neutral mutants are similar to the wild type proteins with respect to brightness and emission wavelength.