Table 1.
Progesterone concentration (mM) | Average diameter of colonies incubated at 25°C (cm)a,b,c | Average diameter of colonies incubated at 35°C (cm)a,b,c |
---|---|---|
0 |
2.40 ± 0.18 |
1.47 ± 0.13 |
0.010 |
2.35 ± 0.10 |
1.33 ± 0.11 |
0.050 |
2.10 ± 0.11* |
no growth |
0.125 |
1.78 ± 0.07* |
no growth |
0.250 |
1.47 ± 0.16* |
no growth |
0.500 | 1.22 ± 0.11* | no growth |
This table shows the colony diameter attained after conidia were inoculated at 25°C and 35°C in a modification of medium M agar plates with different concentrations of added progesterone. No growth was observed at concentrations of progesterone of 0.05 mM or above, at 35°C while conidia incubated at 25°C germinated and showed growth at all concentrations of progesterone tested. The data represents the average diameter ± one std deviation of 6 independent experiments.
a The cultures were incubated at the desired temperature for 20 days.
b All cultures were inoculated with 5μl of a suspension containing 106/μl conidia.
c The values given are the average of 6 independent determinations.
* The values marked with an asterisk are significantly different from the values where no progesterone was added to the medium.