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Comparison of PLP tautomeric forms of wild-type CBS and Thr-257 mutants. Fluorescence emission spectra of 40 μm wild-type CBS (A) in 100 mm phosphate buffer, pH 8.0, and of the T257M (B), T257I (C), T257S (D), and T257V (E) CBS mutants. The excitation wavelength was 330 nm.
