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Comparison of PLP tautomeric forms of wild-type CBS and Thr-260 mutants. The fluorescence emission spectra are shown for 40 μm wild-type CBS (A) in 100 mm phosphate buffer, pH 8.0, and of the T260M (B), T260S (C), and T260V (D) CBS mutants. The excitation wavelength was 330 nm.
