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Effect of heme reduction and carbonylation on CBS activity
| CBS | Specific activity (+PLP, +AdoMet) |
|||
|---|---|---|---|---|
| Ferric | Ferrous | Ferrous-CO | Reoxidizeda | |
| μmol/h/mg protein | ||||
| Wild type | 491 ± 9 | 241 ± 34 | 51 ± 5 | 457 ± 16 |
| T257M | 5 ± 1 | NDb | ND | ND |
| T257I | 3 ± 0.5 | 0.4 ± 0.2 | ND | ND |
| T257S | 300 ± 40 | 166 ± 12 | ND | 124 ± 14 |
| T257V | 39 ± 6 | 4 ± 1 | ND | ND |
| T260M | 168 ± 12 | 28 ± 8 | ND | 9 ± 1 |
| T260S | 580 ± 60 | 284 ± 10 | 21 ± 2 | 116 ± 16 |
| T260V | 264 ± 35 | 130 ± 16 | ND | 40 ± 12 |
a Ferrous-CO samples that were air-oxidized.
b ND, not detectable.
