FIGURE 2.

Detection of dynamin isoforms 1–3 within mouse testis. Mouse testis sections were subjected to immunolocalization with antibodies against dynamin isoforms 1 (A–D), 2 (E–H), and 3 (I–L) (green) and counterstained with the nuclear dye, propidium iodide (red). Dynamin isoforms 1 and 2 (A–H) localized to a structure within maturing germ cells that coincides with the proacrosomal vesicle (A and E; arrow), showing the characteristic flattening as the germ cells mature (B–D and F–H). A less intense nonacrosomal labeling was also observed for dynamins 1 and 2 proximate to a number of early germ cells (A and E; arrowhead). Dynamin 3 displayed minimum punctate labeling throughout the developing germ cells. The images are representative of entire testis section. Scale bars,100 μm for A, E, and I; and 7 μm for B–D, F–H, and J–L.