FIGURE 5.

Examination of the localization of dynamin isoforms in mature cauda epididymal spermatozoa. Antibodies against dynamin isoforms 1, 2, and 3 were used to determine the localization of the three proteins (green), and these cells were then counterstained with the TRITC-conjugated (red) acrosomal marker, PNA. Both dynamin isoforms 1 and 2 (A and B, respectively; green) were strongly colocalized with PNA within the acrosomal region. In contrast, dynamin 3 (C) was not able to be detected in these cells. Representative images are shown. Scale bars, 10 μm.