FIGURE 6.
Investigation of the effect of pharmacological inhibition of dynamin on the ability of mouse spermatozoa to engage in acrosomal exocytosis. Mouse spermatozoa were either noncapacitated (Non-Cap.), capacitated (Cap.), or capacitated in the presence of two different inhibitors of dynamin 1 and 2 (dynasore and Dyngo-4a). Each inhibitor was used at three different concentrations, namely 1, 10, and 100 μm. Viable cells were recorded as acrosome reacted upon the loss of PNA staining when viewed by fluorescence microscopy. A, there was no reduction in the number of acrosome-reacted cells at any concentration of inhibitor in the 2.5 μm A23187-induced treatments when compared with Me2SO vehicle control (DMSO). B, there was, however, a significant (P < 0.05) reduction in the number of acrosome-reacted cells for all treatments at medium and high inhibitor concentrations when the progesterone-induced (15 μm) acrosome reaction was assayed. Each experiment was replicated three times, and the results are presented as the means ± S.E. *, P < 0.05; **, P < 0.01; ***, P < 0.001.