FIGURE 5.
Dab2 SBM precludes sulfatide-mediated platelet aggregation. Representative time series of images of test microchannels with platelets and aggregates under physiological flow conditions are indicated on top. Washed platelets loaded without (A) and with (B) sulfatide liposomes were preincubated with the following proteins/peptides (10 μm each): Dab2 N-PTB (C), Dab2 N-PTB4M (D), Dab2 SBM (E), and RGDS (F). Images were taken 0, 3, and 10 min after the start of flow. The last column on the right illustrates how unadhered objects (green), isolated platelets (white), and platelet aggregates (red) are visualized in the channel. Scale bar, 45 μm; arrows indicate flow direction. Representative of n = 3 experiments. Lipo-S, sulfatide-enriched liposomes.